The impact of anatomical resection for hepatocellular carcinoma with microvascular invasion on early tumor recurrence / 中华肝胆外科杂志

Objective To study the impact of anatomical resection (AR) for hepatocellular carcinoma with microvascular invasion on early tumor recurrence.Methods 178 consecutive patients with solitary hepatocellular carcinoma who underwent liver resection at the Affiliated Tumor Hospital of Guangxi Medical University from January 2012 to December 2015 were retrospectively studied.These patients were divided into four groups according the types of resection (anatomical resection AR or non-anatomical resection NAR) and the surgical margins (narrow or wide margin).All the patients were followed up until the tumor recurred or two years after surgery.The recurrence-free survival and the types of recurrence for the 4 different groups were compared.Results The 6 months,1-,2-year disease-free survival rates in the AR (n =55) and NAR groups (n =123) were 87.0％,79.2％,74.5％ and 78.5％,61.3％,45.7 ％,respectively,(P ＜ 0.05).Through pair-wise comparisons of the four groups,there were no significant differences in early recurrence between the narrow surgical margin group and the wide surgical margin group,regardless of the types of surgery.The early recurrence rates of the AR groups were significantly lower than that of the NAR groups,regardless of the widths of the surgical margins.Multivariate analysis showed that AR was a protective factor of early recurrence (HR =0.417,95％ CI 0.229 ～ 0.761).Further analysis of the recurrence patterns of AR and NAR showed that the recurrence types were mainly sohtary for AR (solitary,61.5％;multiple 30.8％) and multiple recurrence for NAR (solitary,32.2％;multiple 61.0％).Conclusion AR improved early recurrence-free survival of patients with microvascular invasion.

COMPARISON OF IMMUNOENZYMATIC STAINING TECHNIQUE (IEST), DOUBLE GLUING STRIP CIRCUMOVAL PRECIPITIN TEST(DGS-COPT) AND CONVENTIONAL CIRCUMOVAL PRECIPITIN TEST(CV-COPT) FOR DIAGNOSIS OF SCHISTOSOMIASIS JAPONICA / 中国寄生虫学与寄生虫病杂志

IEST, DGS-COPT and CV-COPT using lyophilized ova of schistosoma japonicum were performed on sera from 120 cases of schistosomiasis japonica, 120 cases of schis-tosomiasis japonica 3-8 years after being cured with praziquantel and 120 healthy individuals by single-blind method. The sensitivity and specificity of IEST was 91.7% and 95.8% respectively which were significantly higher than that of both DGS-COPT and CV-COPT. The negative conversion rate of cured patients was 70.8% with IEST, 80.8% with DGS-COPT and 81.7% with CV-COPT. The results showed that IEST has higher diagnostic value for schistosomiasis than both COPT. DGS-COPT has the same diagnostic value as CV-COPT, however, it was easy to perform and time-saving, thus it might be applied in the fields for practical purposes.

DETECTION OF ENZYME DISSOCIATED SCIC FROM SERA OFSCHISTOSOMIASIS JAPONICA BY TWO-DIRECTION COUNTER.IMMUNOELECTROPHORESIS / 中国寄生虫学与寄生虫病杂志

A new method of two-direction counter-immunoelectrophoresis (TD-CIEP and TD-ELACIEP) for detection of specific antigen and antibody of enzyme-dissociated schisto-some circulating immune complexes (SCIC) in infected rabbits and advanced schistosomia-sis cases was reported. Results showed that the detection rate was markedly increased in the enzyme-dissociated sera as compared with that of non-dissociated sera. The levels of antigen and antibodies were found to be correlated to the period and intensity of infection. The detected antigen was solely originated from the adult worm. Comparison of sensitivity of both methods showed that TD-ELACIEP was much more sensitive than TD-CIEP either for detection of antigen or antibodies. Of 54 sera of advanced schistosomiasis cases the detection rate of schistosome antigen or antibodies by TD-ELACIEP in enzyme-dissociated and non-dissociated serum samples was 50 and 25.9%, respectively. Only 9 cases( 16.7%) were COP positive with COP rate

STUDIES ON THE DIAGNOSIS OF HUMAN FILARIASIS BY INDIRECT FLOURESCENT ANTIBODY TEST(IFAT) AND IMMUNOENZYMATIC STAINING TECHNIQUE(IEST) WITH HOMOLOGOUS AND HETEROLOGOUS ADULT FILARIAL ANTIGENS / 中国寄生虫学与寄生虫病杂志

This article deals with the diagnostic values of IFAT and IEST in human filariasis with both frozen sections of Brugia malayi and Setaria cervi adult worms as antigens. The average positive and the false positive rates of IFAT with two antigens were 84.9-97.1%(45/53-102/105)and 2.9-9.7%(1/35-3/31) respectively, while no cross-reaction was observed in subjects infected with ascaris or hookworm. The average positive and the false positive rates of IEST with two antigens were 94.3-94.9% (99/105-56/59) and 0-2.9% (0/35-l/35) respectively. Both IFAT and IEST with two antigens for the diagnosis of human filariasis were considered to be of higher sensitivity and specificity, frozen sections of adult Setaria cervi being more economical and effective.